Composite

Part:BBa_K737043

Designed by: Wenjie Wu and Peiran Zhang   Group: iGEM12_OUC-China   (2012-09-19)


constitutive Spot42 generator ligated to lacI generator

[J23106,P0412]is a LacI generator, which represses tet promotor and can be induced by IPTG. aTc concentration gradient results in different sRNA Spot42 levels in cells when presented R0011, which can be monitored by our galK::GFP generator,where galK is the RBS of GFP and the target for Spot42. This device was mistaken for aTc inducible sRNA device originally due to some ligation mistake,but we actually found this one is perfect to express full level sRNA to verify sRNA function without induction. When assembly the corresponding part together(constitutive Spot42 generator ligated to TetR generator),the double input platform will work efficiently.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aconstitutive Spot42 generator ligated to lacI generator